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Image Search Results
Journal: Nutrients
Article Title: Dietary Soy Protein Isolate Attenuates Intestinal Immunoglobulin and Mucin Expression in Young Mice Compared with Casein
doi: 10.3390/nu12092739
Figure Lengend Snippet: Primers for qRT-PCR.
Article Snippet: The following antibodies were used: anti-Janus kinase-1 (JAK1) (bs-1439R, Bioss, Beijing, China), anti-phospho (p)-JAK1 (Tyr1034 + Tyr1035, bs-3238R, Bioss, China), anti- signal transducer and activator of transcription 6 (STAT6) (380957, ZEN BIO, China), anti-p-STAT6 (Tyr641, ab263947, Abcam, Cambridge, MA, USA), anti-pIgR (AF2800-SP, R&D Systems, Minneapolis, MN, USA),
Techniques:
Journal: Nutrients
Article Title: Dietary Soy Protein Isolate Attenuates Intestinal Immunoglobulin and Mucin Expression in Young Mice Compared with Casein
doi: 10.3390/nu12092739
Figure Lengend Snippet: Effect of SPI on JAK1-STAT6 signaling and TGFβ levels in mouse ileum tissue. ( A ) qRT-PCR analysis of Jak1 , Stat6 , Tgfb1 , and Tgfb2 mRNA expression levels in mouse ileum tissue (normalized against Actb (β-actin); n = 8). ( B ) Western blotting analysis of JAK1, p -JAK1, STAT6, p -STAT6, pIgR, and TGFβ1 protein levels in mouse ileum tissue ( n = 6). ( C ) The statistical analyses of the Western blotting results. Results are normalized to β-actin ( n = 6). All data are presented as means ± SEM. NS, not significant ( p > 0.05); * p < 0.05; ** p < 0.01. Statistical significance was calculated using t -tests.
Article Snippet: The following antibodies were used: anti-Janus kinase-1 (JAK1) (bs-1439R, Bioss, Beijing, China), anti-phospho (p)-JAK1 (Tyr1034 + Tyr1035, bs-3238R, Bioss, China), anti- signal transducer and activator of transcription 6 (STAT6) (380957, ZEN BIO, China), anti-p-STAT6 (Tyr641, ab263947, Abcam, Cambridge, MA, USA), anti-pIgR (AF2800-SP, R&D Systems, Minneapolis, MN, USA),
Techniques: Quantitative RT-PCR, Expressing, Western Blot
Journal: Frontiers in Immunology
Article Title: Higher density of CD4+ T cell infiltration predicts severe renal lesions and renal function decline in patients with diabetic nephropathy
doi: 10.3389/fimmu.2024.1474377
Figure Lengend Snippet: Immunostaining results of some molecules among different groups. (A–L) Immunohistochemical results of Bcl6 (A–C) , IL-6 (D–F) , STAT3 (G–I) , and TGFβ1 (J–L) in the high-CD4, low-CD4, and non-diabetic nephropathy (NDN) groups (scale bar = 100 μm; red arrows indicate partial positive expression). (M–P) The expression of Bcl6, IL-6, STAT3, and TGFβ1 in renal tissue was more obvious in the high-CD4 group than in the low-CD4 and NDN groups (data was shown as mean ± SD, five cases in each group; ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001).
Article Snippet: Immunostaining for Bcl6 (1:100, 66340-1-LG, Proteintech), IL-6 (1:200, GB11117, Servicebio), STAT3 (1:100, ab68153, abcam), and
Techniques: Immunostaining, Immunohistochemical staining, Expressing